## **Introduction**

**Sequence Space** is a gene analysis platform developed using *`HTML`*, *`CSS`*, and *`JavaScript`**,*** designed to challenge the common perception that bioinformatics platforms are merely functional and often lack creativity and aesthetics. The website features a clean, minimalist design that balances functionality with visual appeal.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F4fdbf807-c3c2-430c-b213-df839c97cc01.mp4?alt=media&token=bc1c5906-7634-4724-9fae-dc733d08aff3","id":"4fdbf807-c3c2-430c-b213-df839c97cc01","width":3024,"height":1786,"filename":"new.mp4","type":"video/mp4","caption":"","border":false}]}
```

## **Aims and Desired Directions**

The **Sequence Space** project aims to develop a user-friendly web interface designed to cater to various DNA sequence analysis needs. This platform provides tools to detect palindromic sequences, which are crucial in understanding genomic structures, and offers additional basic gene-related services.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F802007fb-367f-42d7-987d-0d9b76b50422.mp4?alt=media&token=79b04e5a-ba8a-469d-bc1b-7c59780877e8","id":"802007fb-367f-42d7-987d-0d9b76b50422","width":1080,"height":720,"filename":"Showreel_-Web-gallery-[remix].mp4","type":"video/mp4","caption":"","border":false}]}
```

Key functionalities include palindrome detection and basic gene sequence analysis tools. The platform is responsive and accessible, aiming to provide essential DNA analysis tools in a user-friendly interface that is both engaging and visually attractive. **Sequence Space** demonstrates that bioinformatics platforms can be both functional and aesthetically pleasing.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F506f7474-d6a8-4854-ac89-9f1f84901df9.png?alt=media&token=6b681255-3077-4d9b-a34b-db4e413f62d5","id":"506f7474-d6a8-4854-ac89-9f1f84901df9","width":3022,"height":1794,"filename":"Screenshot 2024-08-23 at 11.36.35 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F38d53383-266f-4695-b0ea-170aa73dfb09.png?alt=media&token=f3608d27-56ee-4334-97d9-ed861925134f","id":"38d53383-266f-4695-b0ea-170aa73dfb09","width":3024,"height":1736,"filename":"Screenshot 2024-08-23 at 4.36.41 PM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2Fc1e2cbfd-3e97-4996-9b64-6ba091e9bae1.png?alt=media&token=0d94ce88-6106-4bb7-9149-b50e42d30898","id":"c1e2cbfd-3e97-4996-9b64-6ba091e9bae1","width":3024,"height":1780,"filename":"Screenshot 2024-08-23 at 11.34.51 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F5be50a78-bf93-43ac-91d5-b698706610c4.png?alt=media&token=259ec470-c041-4c06-9571-0f3954cd4ec2","id":"5be50a78-bf93-43ac-91d5-b698706610c4","width":3024,"height":1778,"filename":"Screenshot 2024-08-23 at 4.40.19 PM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F609a2a22-1d9c-4e01-b9c7-f9d07f853e13.png?alt=media&token=df0511ba-7a8e-47e6-8c4f-b7aebd71b44e","id":"609a2a22-1d9c-4e01-b9c7-f9d07f853e13","width":3024,"height":1788,"filename":"Screenshot 2024-08-23 at 4.50.59 PM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fseq-space%2F101548a5-b070-4cb8-9e0b-8b554442094e.png?alt=media&token=73cc3791-7905-444f-bd9a-c99d75fdcce0","id":"101548a5-b070-4cb8-9e0b-8b554442094e","width":2802,"height":1786,"filename":"Screenshot 2024-08-23 at 4.35.29 PM.png","type":"image/png","caption":"","border":false}]}
```

Built using *HTML*, *CSS*, and *JavaScript*, the website ensures an intuitive and responsive user experience, enabling researchers and genetic enthusiasts to easily explore and analyze DNA sequences. The primary goal of **Sequence Space** is to make advanced genetic analysis accessible to a broader audience by providing a streamlined and efficient platform for DNA sequence examination.

Sequence Space: Website Interface for Gene Analysis Platform

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite%2F505ece23-9ab8-4ab6-8ed3-93f7f0eaa656.mov?alt=media&token=0a565294-2393-4b48-9538-affefaef0b4a","id":"505ece23-9ab8-4ab6-8ed3-93f7f0eaa656","width":1080,"height":1052,"filename":"BF77B7B5-C4B0-45A8-A8C7-822BB6E645EB.mov","type":"video/quicktime","caption":"","border":false}]}
```

I appreciate what WakeWhite shared with me:

### **'We trust your aesthetic.**

### **We love your style.**

### **Just do whatever you want.**

### **Follow your heart.'**

Hence, I am proud to announce our collaboration with WakeWhite, a partnership that evolved organically as they witnessed the excellence of our previous photography work.

---

### **Indoor Elegance**

Within the confines of carefully selected indoor settings, I made my best effort to capture the essence of WakeWhite's products. Every shot reflects the sophistication and refinement that lie at the heart of the brand.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2FOi11PG7fU4M1oNiljDS5%2Fe1cc90f2-55bb-43e5-a343-aec29b02181d.jpg?alt=media&token=b9dc0228-d02c-40df-aa79-5f29b3da76fb","id":"e1cc90f2-55bb-43e5-a343-aec29b02181d","width":3024,"height":4032,"filename":"9EA5C871-C1BF-4826-9B79-D378389D9420.JPG","type":"image/jpeg","caption":"Shots by Siang Hooi","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2FOi11PG7fU4M1oNiljDS5%2F1e826008-f972-4b10-bf88-f5b971585ff0.jpg?alt=media&token=fab66b39-8cac-40a3-93e8-96dcb28fe000","id":"1e826008-f972-4b10-bf88-f5b971585ff0","width":3024,"height":4032,"filename":"B46CF136-B846-4CBD-BA27-1389E1D76C38.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2FOi11PG7fU4M1oNiljDS5%2F1404329a-b915-4261-a981-53fa294ee365.jpg?alt=media&token=ff774e23-946b-4fd4-a775-eaecab7c8174","id":"1404329a-b915-4261-a981-53fa294ee365","width":3006,"height":3683,"filename":"CA2DE0F7-597A-479E-8A08-739ADC2DE6A9.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite%2F41758a74-e0d4-4fb8-99ad-21d96fc271e6.jpg?alt=media&token=22f34f53-78ae-4e65-bd1a-dcdbb34c6cfe","id":"41758a74-e0d4-4fb8-99ad-21d96fc271e6","width":1290,"height":1728,"filename":"7262A38A-552C-4C4A-A46A-0E3A31DFD65A.jpeg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2FOi11PG7fU4M1oNiljDS5%2Fb3bf4a6b-36fb-402b-9ff2-4b29d68e9962.jpg?alt=media&token=ad88e7f6-3752-4cf1-aa5d-f09601867167","id":"b3bf4a6b-36fb-402b-9ff2-4b29d68e9962","width":1030,"height":1288,"filename":"E214C755-705F-4FDE-A30D-551B7891D280.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite-projects%2F45bfbb8d-fb7d-47fc-bdb1-efe7d6709f74.jpg?alt=media&token=ee992853-c906-4de5-95fe-b978f02563aa","id":"45bfbb8d-fb7d-47fc-bdb1-efe7d6709f74","width":1290,"height":1755,"filename":"6DF43B7F-1808-4551-91D3-D3C51F0D6440 2.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite-projects%2Fd5f6097b-85ea-4ff8-aaaf-4cc91c3304ab.jpg?alt=media&token=5a5e9ba8-fc43-4d77-989c-9edc2350bf5f","id":"d5f6097b-85ea-4ff8-aaaf-4cc91c3304ab","width":3024,"height":4032,"filename":"5F2D9F9C-1052-4EA4-9582-D14EFD4BD62F.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite%2Fef327d64-9788-4c37-bb1b-6e2e04c587c7.jpg?alt=media&token=d176bc90-d21e-4b15-8359-6fe0dead253a","id":"ef327d64-9788-4c37-bb1b-6e2e04c587c7","width":1290,"height":1678,"filename":"6B4925CD-1ED3-48A1-BD86-36AA4847B9A9.jpeg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite-projects%2Faf80c053-5273-49d6-9340-bc4cc2568073.jpg?alt=media&token=6f518c51-e0c9-43ae-b139-90e489179ef8","id":"af80c053-5273-49d6-9340-bc4cc2568073","width":3219,"height":4032,"filename":"B0EB8559-38E0-4ECA-B988-A8F7C18FEB35.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fwakewhite%2Feb7c6d9c-8d9b-4e55-b4cb-155d9cf074c7.jpg?alt=media&token=c4cd40e1-e114-4e39-813a-20ede0a38a53","id":"eb7c6d9c-8d9b-4e55-b4cb-155d9cf074c7","width":1290,"height":1678,"filename":"DF7E57B5-5FBB-4CE6-BEE5-75FAA305D775.jpeg","type":"image/jpeg","caption":"","border":false}]}
```

### **Outdoor Enchantment**

Stepping outdoors, I embraced the natural world as my canvas. The outdoor shots not only highlighted the products' adaptability but also showcased their allure against the backdrop of the real world. These shots resonate with the harmonious coexistence of beauty and nature that WakeWhite stands for.

---

### **The Journey of Beauty**

I am delighted to share that these compelling visuals now hold a prominent place on WakeWhite's official website. This collaboration is a testament to our dedication to presenting products in the best light, reflecting the essence of WakeWhite's mission: enhancing beauty through the power of nature.

### **" Explore our journey with WakeWhite and experience beauty redefined. "**

---

### **Credits**

Photographer/ Videographer: Siang Hooi\
Model: Shereen Wee\
Edits: Siang Hooi

Device: iPhone 13 Pro Max\
Applications: VN, VSCO, Unfold, Adobe Lightroom

Collaborative Journey with WakeWhite

## **Overview**

**Objective**: To conceptualize a precision agriculture system for identifying and managing weeds in paddy fields, focusing on interface design and system lifecycle planning.

**Scope**: The project was theoretical, emphasizing system design, documentation, and user interface prototyping rather than building a working system.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fda1aa9fa-7508-4d4d-9683-854fd92ab381.png?alt=media&token=3271f55d-f056-46a1-b4db-c7a35607122d","id":"da1aa9fa-7508-4d4d-9683-854fd92ab381","width":1920,"height":1080,"filename":"12.png","type":"image/png","caption":"","border":true},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fc0dfcb82-a294-4148-bd9c-342df9a65e9d.png?alt=media&token=3b21d1e4-a0b3-42c0-bb51-fd6b78cafe6f","id":"c0dfcb82-a294-4148-bd9c-342df9a65e9d","width":1920,"height":1080,"filename":"11.png","type":"image/png","caption":"","border":true},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fccfbce5c-4366-4fb5-9b0d-4d3ba9a960c2.png?alt=media&token=0adf5999-41f9-4f8e-b09f-06ddc14171f5","id":"ccfbce5c-4366-4fb5-9b0d-4d3ba9a960c2","width":1920,"height":1080,"filename":"9.png","type":"image/png","caption":"","border":true}]}
```

---

## **Methodology**

### ***Software Development Life Cycle (Waterfall Approach)***

1. ***Requirement Gathering***

   • Conducted stakeholder interviews and surveys to identify system needs and challenges.

   • Focused on designing features like weed detection mapping, user-friendly reporting, and data integration.

2. ***System Design***

   • Created use case diagrams, context diagrams, Level 0 and Level 1 data flow diagrams, and conceptual architecture using **Lucidchart**.

   • Developed functional decomposition for each module, ensuring clarity and modularity.

3. ***Interface Prototyping***

   • Designed user registration, dashboards, report generation, and tutorial interfaces using **Figma**, focusing on usability and ease of navigation.

4. ***Phased Implementation Strategy***

   • Outlined a step-by-step deployment plan from data collection to full system functionality.

5. ***Documentation***

   • Prepared detailed system requirement specifications, decision tables, event-response tables, and structured English for process clarity.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fca9905ee-0a98-425f-907e-e67dd407c854.png?alt=media&token=730aefb2-874a-43c6-8183-507b8bd593dd","id":"ca9905ee-0a98-425f-907e-e67dd407c854","width":1406,"height":1260,"filename":"Screenshot 2024-12-27 at 12.30.25 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fd183097d-8147-4369-a27f-3af2f1b041ca.png?alt=media&token=5a46f3c4-94d4-4210-ad85-2df4b3325695","id":"d183097d-8147-4369-a27f-3af2f1b041ca","width":1470,"height":756,"filename":"Screenshot 2024-12-27 at 12.31.13 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2F7d92bdd3-d8d2-4e4d-9a78-df0be4a1a86c.png?alt=media&token=77a8a3f1-98ea-4288-ae94-2fb6432a74e7","id":"7d92bdd3-d8d2-4e4d-9a78-df0be4a1a86c","width":1482,"height":726,"filename":"Screenshot 2024-12-27 at 12.31.26 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2F732ce0d9-3792-4a2d-9208-8478a553507e.png?alt=media&token=73fa5b11-eac6-457d-9bba-b520e03506b9","id":"732ce0d9-3792-4a2d-9208-8478a553507e","width":844,"height":1462,"filename":"Screenshot 2024-12-27 at 12.31.44 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fe58f00da-f4e2-4fea-afc7-db0d82cfbab3.png?alt=media&token=88baff42-f87e-4b01-84ba-f96ed296ae84","id":"e58f00da-f4e2-4fea-afc7-db0d82cfbab3","width":1558,"height":838,"filename":"Screenshot 2024-12-27 at 12.31.58 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2Fe418de2a-5427-4c87-ab83-f19ca7e3feb0.png?alt=media&token=a70db663-1e86-45a3-a1c1-9f6b3fa31b8a","id":"e418de2a-5427-4c87-ab83-f19ca7e3feb0","width":772,"height":1064,"filename":"Screenshot 2024-12-27 at 12.32.19 AM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2F8a3417c2-9562-434b-a823-5378b7084202.png?alt=media&token=96bde351-5a01-4c47-ac35-6d848fdb4449","id":"8a3417c2-9562-434b-a823-5378b7084202","width":828,"height":312,"filename":"Screenshot 2024-12-27 at 12.32.28 AM.png","type":"image/png","caption":"","border":false}]}
```

---

## **Key Deliverables**

### **Figma Mockups:**

* **Landing Page**: A simple gateway for user login and registration.

* **User Registration**: Multi-step registration with email verification.

* **Dashboard**: Centralized interface for accessing reports and tutorials.

* **Reports Page**: Conceptualized data visualizations for weed detection insights.

* **Feedback & Tutorial Pages**: Designed for user support and engagement.

```cv
gallery
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```

---

## **Focus Areas**

1. **Usability**: Designed for farmers with varying technical expertise, prioritizing clear navigation and user-friendly processes.

2. **Sustainability**: Conceptualized features to reduce herbicide usage and promote environmentally friendly practices.

3. **Stakeholder-Centric Design**: Integrated insights from farmers, researchers, and environmental experts to address real-world needs.

---

## **Outcome**

1. Delivered a well-documented, user-focused conceptual system with high-quality prototypes and diagrams.

2. Demonstrated a structured approach to system development and highlighted its potential impact on sustainable agriculture.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fagroplus-weed%2F9b5ada7e-4b6f-4383-a453-26d9ec8bec84.png?alt=media&token=42474eb9-4f5e-4703-97a4-95b81c2543f5","id":"9b5ada7e-4b6f-4383-a453-26d9ec8bec84","width":1920,"height":1080,"filename":"13.png","type":"image/png","caption":"","border":false}]}
```

AgroPlus Weed Detection System

## **Title:**

### Investigation Of The Genetic Polymorphism And Erythrocyte Binding Activity Of The Plasmodium knowlesi Duffy Binding Protein Alpha Region II (*PkDBPαII*) From Isolates In Malaysia

**Created by** Khor Siang Hooi

**Supervised by** Dr. Cheong Fei Wen, Prof. Fong Mun Yik

---

## **Background**

> This research investigates the genetic diversity and erythrocyte binding activity of ***Plasmodium knowlesi*** **Duffy Binding Protein Alpha Region II (*PkDBPαII*)**, particularly focusing on samples collected from various regions of Malaysia between 2019 and 2021.
>
> ***Plasmodium knowlesi***, a monkey malaria parasite, is currently the most significant cause of human malaria infections in Malaysia, with Malaysian Borneo reporting the highest case numbers. This study focuses on understanding the genetic polymorphisms of *PkDBPαII* and its ability to bind to human red blood cells, which is crucial for the malaria parasite to invade human hosts.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2F2dafbd8e-df51-40f3-bb9b-fe7ce9a947ba.jpg?alt=media&token=5757cc94-49e3-451a-aa9b-0e9c97dcb139","id":"2dafbd8e-df51-40f3-bb9b-fe7ce9a947ba","width":5000,"height":3600,"filename":"national-institute-of-allergy-and-infectious-diseases-Ykv2gm43QFc-unsplash.jpg","type":"image/jpeg","caption":"The interaction between parasite ligand DBL and DARC receptor on erythrocytes","border":true}]}
```

## **The study has two main objectives:**

### **1. To evaluate the genetic polymorphisms of *PkDBPαII* in *P. knowlesi* isolates:**

The study aims to assess genetic variations in *PkDBPαII* among recent *P. knowlesi* isolates collected from Malaysia between 2019 and 2021. Ten isolates are randomly selected from both **Peninsular Malaysia (n=5) and Malaysian Borneo (n=5)**, representing a mix of high and low parasitemia blood samples.

To achieve this objective, the employed techniques involve PCR to amplify *PkDBPαII*, followed by DNA cloning into the pGEM-T vector. Subsequently, two positive recombinant clones were picked from each sample and conducted sequencing. The collected data were then utilised to construct a phylogenetic tree using MEGA X. Comparing these sequences to earlier ones from **Fong et al.'s studies (2014; 2015)**, the analysis presented **all 20 sequences fell into two allele groups**, consistent with previous research.

### **2. To investigate the erythrocyte binding activity of *PkDBPαII* in isolates with different parasitemia & geographical regions:**

It involves studying how *PkDBPαII* from these isolates binds to human erythrocytes. For that, four isolates were selected. I first digested pGEM-T-PkDBPαII recombinant plasmids using the *Bgl*II restriction enzyme and cloned them into the pDisplay-AcGFP1 vector. The resulting constructs were sent for sequencing.

The next step involves transfecting these recombinant plasmids into COS-7 cells before conducting an erythrocyte binding assay (EBA). During EBA, human erythrocytes were added to the transfected COS-7 cells, stained with Hoechst 33342 dye, and observed under a fluorescence microscope. **Rosettes, defined as aggregates of erythrocytes adhering to more than 50% of COS-7 cells, were counted,** and their **sizes** were determined using imaging software. The statistical analysis is planned using IBM SPSS version 22.

### 

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2F8375d8f2-8f48-4f9e-9a48-66d678da4669.jpg?alt=media&token=4f3ead75-9ad4-4771-a283-b8882e08b51f","id":"8375d8f2-8f48-4f9e-9a48-66d678da4669","width":2832,"height":3920,"filename":"cdc-wDxFn_dBEC0-unsplash.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2F34eb5342-636f-43b1-934d-209fc4b27e7b.jpg?alt=media&token=b426bc0e-46a0-4b11-8129-3739de53fc23","id":"34eb5342-636f-43b1-934d-209fc4b27e7b","width":4640,"height":2610,"filename":"dinesh-jatav-xHz4_cOube0-unsplash.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2F3824b861-3ab2-4cd4-b3c9-26c897d2d706.jpg?alt=media&token=f890932b-ca25-4c96-921d-31c04f7286b3","id":"3824b861-3ab2-4cd4-b3c9-26c897d2d706","width":4000,"height":6000,"filename":"paul-einerhand-bKd9KEELfmg-unsplash.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2Fe09cc300-59d8-44fb-9f4b-ba4b83e0c414.jpg?alt=media&token=af917214-b579-4788-b956-22f5d91e70e4","id":"e09cc300-59d8-44fb-9f4b-ba4b83e0c414","width":3456,"height":5184,"filename":"national-cancer-institute-jeT_DE3esSI-unsplash.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2F0e8f6cd9-5adb-4420-9fbf-5662b0c5d6f0.jpg?alt=media&token=d1a586ba-f89a-48fb-8260-b1e6a1c7f075","id":"0e8f6cd9-5adb-4420-9fbf-5662b0c5d6f0","width":2832,"height":3965,"filename":"jaron-nix-5HbxyB0_DBg-unsplash.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmalaria-pkdbp-fyp%2Fd593b1ad-bbe7-4042-a237-36e1a2f09ecf.jpg?alt=media&token=1ccc1a29-c483-43bc-baba-a0f5b9ef51f4","id":"d593b1ad-bbe7-4042-a237-36e1a2f09ecf","width":5184,"height":3456,"filename":"national-cancer-institute-fd0b-Bl4cFc-unsplash.jpg","type":"image/jpeg","caption":"","border":false}]}
```

## **Methodology:**

* **Cloning of PkDBPαII** (PCR, colony PCR, plasmid extraction)

* **Cloning into the pDisplay-AcGFP1 vector** (RE *Bgl*II digestion, directional PCR)

* **Maintenance of COS-7 cells** (thawing, subculture, cryopreservation)

* **Erythrocyte Binding Assay** (plating, transfection, staining, rosette analysis)

* **Gene sequencing & analysis; Phylogenetic study** (UGENE, MEGA X)

## **Conclusion:**

From what has been presented in the phylogenetic trees of the current study, there has been **no novel genetic clustering** of *P. knowlesi*-infected *PkDBPαII* isolates in recent years, particularly **from 2019 to 2021**. These findings highlight the importance of further investigation into how these **genetic variations** might influence malaria transmission and severity.

The study also sets the groundwork for future research that can explore potential vaccine targets or therapeutic interventions by analyzing more samples and additional factors, such as environmental influences on parasite transmission.

Have Malaria Parasites Evolved Over the Recent Years?

[View Full-text Thesis](https://drive.google.com/file/d/1TquIC6Jtx9aETcP7ERWq4oTqyiKjg380/view?usp=share_link)

[View Presentation Version](https://drive.google.com/file/d/1AwJJiKHF4eln5nS3uXYm0DLEamBsI1Qn/view?usp=share_link)

---

## **Background**

**Cardiovascular diseases (CVDs)** are the leading cause of death globally, with myocardial infarction (MI) being a major contributor. My project uses advanced **deep-learning** techniques to improve early MI detection from echocardiograms. By integrating **U-NET** for image segmentation and **Long Short-Term Memory (LSTM)** networks with **Global Longitudinal Strain (GLS)** parameters, this approach aims to enhance diagnostic accuracy and reduce the reliance on manual interpretation.

## **Problem Statement**

Early and accurate MI detection remains a significant challenge. Traditional methods, like manual interpretation of echocardiograms, can be inconsistent. Conventional metrics like ejection fraction (EF) often miss early signs of heart issues. My solution addresses these limitations using GLS, a more sensitive measure of heart function, integrated with a deep learning model.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fb8fx5RY9kN0ZahlLQyYN%2Fcace5b87-9452-4cc5-9b0e-460331a7c861.png?alt=media&token=70ae731a-553e-4337-a8bf-22b49b458920","id":"cace5b87-9452-4cc5-9b0e-460331a7c861","width":2138,"height":1364,"filename":"Screenshot 2024-10-13 at 4.16.58 PM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fb8fx5RY9kN0ZahlLQyYN%2F9f097f25-b04f-40d2-84fc-6d00334095f5.png?alt=media&token=830781b9-df03-43e6-9efa-dbb6b31dce5d","id":"9f097f25-b04f-40d2-84fc-6d00334095f5","width":1124,"height":1250,"filename":"Screenshot 2024-10-13 at 4.23.31 PM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fb8fx5RY9kN0ZahlLQyYN%2F8ef0e430-bd2a-4cbd-97d1-19935990c2b0.png?alt=media&token=f8d1db55-60d6-4dec-ad8f-454ec87b2928","id":"8ef0e430-bd2a-4cbd-97d1-19935990c2b0","width":2128,"height":1270,"filename":"Screenshot 2024-10-13 at 4.18.57 PM.png","type":"image/png","caption":"","border":false}]}
```

## **Data Acquisition**

This study utilizes the **HMC-QU dataset**, which was developed by Hamad Medical Corporation, Tampere University, and Qatar University. This dataset includes a collection of two-dimensional (2D) echocardiography recordings in apical four-chamber (AP4) and apical two-chamber (AP2) views, recorded between 2018 and 2019—moreover, additional data from the **Malaysia Hospital UITM (HUiTM) dataset** were added to handle data imbalance.

---

## **What I Did**

I utilized a deep learning model using **U-NET** to segment the left ventricle in apical four-echocardiogram videos, using the HMC-QU dataset and HUiTM dataset. I then extracted **GLS** **parameters** to provide a deeper understanding of heart function. These were integrated into an **LSTM** model, which analyzed the temporal data to predict MI. This novel combination of AI techniques shows promising results for more accurate and reliable MI predictions.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmi-ai%2Fee1a8d71-f8c4-4114-918d-5e451bd0a782.png?alt=media&token=45cc3fd6-d82a-4c7c-b4ce-875742f5fa53","id":"ee1a8d71-f8c4-4114-918d-5e451bd0a782","width":2888,"height":1578,"filename":"Screenshot 2025-01-25 at 2.48.08 PM.png","type":"image/png","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fb8fx5RY9kN0ZahlLQyYN%2F5473efd9-7e6f-4462-87d6-7e9c29c7ac61.jpg?alt=media&token=9da7af98-696b-45e8-b022-6cc6197c0ee3","id":"5473efd9-7e6f-4462-87d6-7e9c29c7ac61","width":358,"height":303,"filename":"B9780323055277500093_gr8.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fb8fx5RY9kN0ZahlLQyYN%2F81483634-25b8-4257-ac92-bb2457dfe777.jpg?alt=media&token=65f87f62-1383-4798-84aa-aec72c2befdf","id":"81483634-25b8-4257-ac92-bb2457dfe777","width":1080,"height":1080,"filename":"New Collection With Laptop Mockup Instagram Post.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fmi-ai%2F1f8d2d4b-8edb-428f-9bc2-1664b9ed4126.jpg?alt=media&token=943c1025-bf36-4e2e-aef4-c77b5f36c038","id":"1f8d2d4b-8edb-428f-9bc2-1664b9ed4126","width":546,"height":413,"filename":"WhatsApp Image 2024-08-13 at 2.37.38 PM.jpeg","type":"image/jpeg","caption":"","border":false}]}
```

## **The Results**

The model achieved **71.5% accuracy** with an **AUC-ROC score of 0.715** in distinguishing MI from non-MI cases. It demonstrated **72.2% precision** and **70.1% sensitivity**. While these results are promising, there's room for improvement in future iterations, particularly to enhance sensitivity.

---

## **Challenges and Future Work**

Although the model performed well, further optimization is needed to improve its clinical utility. Enhancements could include tuning the model’s parameters, adding more features, and validating the results across diverse datasets. This will make the tool even more effective for real-world medical applications.

## **Conclusion**

By integrating **GLS** and **LSTM networks**, I developed a more accurate and consistent method for predicting MI from echocardiograms. This research shows that advanced AI techniques can make a real difference in early heart disease detection, ultimately improving patient outcomes.

Myocardial Infarction Detection using AI

## **Talk about the feelings and thrills**

Working with brands is always fascinating. I fluently juggled my photography style and the expectations of clients. In the creation of the 30-second lifestyle videos, I faced a unique challenge. I had never attempted to shoot a short film before, particularly one set outdoors. It was a daunting task, and I initially lacked confidence.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F38bec5bc-ee0b-4710-8d5e-8f2463bf1cdf.mov?alt=media&token=f9d14656-c90a-48e6-91b9-b1dd22c9a2a8","id":"38bec5bc-ee0b-4710-8d5e-8f2463bf1cdf","width":1080,"height":1074,"filename":"46F841B1-FFE2-4636-BB15-C78EDAE8AC5E 2.mov","type":"video/quicktime","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2Fbc6dc4f7-6dd3-4d04-a954-34ab77e656f4.mp4?alt=media&token=9e4625cf-4bdb-4977-ae5e-240d65de831f","id":"bc6dc4f7-6dd3-4d04-a954-34ab77e656f4","width":1512,"height":2268,"filename":"1696346500432233.MP4","type":"video/mp4","caption":"","border":false}]}
```

However,

I was determined enough to overcome this challenge and committed to delivering the best possible result. As we progressed with the shoot, I gradually gained confidence.

---

## **The New Learning Curve**

Initially hesitant, this project evolved into a fulfilling experience. It reinforced my faith in creativity and storytelling, leaving me with a profound sense of accomplishment.

This journey highlighted the importance of embracing challenges for growth and innovation in visual storytelling. It reminded me that venturing beyond one's comfort zone can lead to exceptional creative achievements.

The unfamiliarity of outdoor shooting and crafting a lifestyle video, which once seemed daunting, transformed into opportunities for creativity. Ultimately, this experience was a testament to the power of pushing boundaries and the rewards that come with embracing the unknown in the pursuit of visual storytelling excellence.

Continuous learning is a positive indication of growth.

---

## **Seeking Inspiration in the Nature**

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F81d32c1f-568e-4598-aef3-85af0f60750a.mp4?alt=media&token=6a52c86c-820a-47f4-b28e-24590f524bb4","id":"81d32c1f-568e-4598-aef3-85af0f60750a","width":1512,"height":1890,"filename":"1696348570207619.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F49740794-f912-4460-90ce-d41696241aa9.mp4?alt=media&token=4cb3119a-e4c6-49e0-b01d-e20e57e53f95","id":"49740794-f912-4460-90ce-d41696241aa9","width":1512,"height":1890,"filename":"1696346765142611.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F0c338da2-8a9d-4bdb-8846-e4cf81e5b463.mp4?alt=media&token=33dfad70-a80b-4d02-bace-e1650255ad6a","id":"0c338da2-8a9d-4bdb-8846-e4cf81e5b463","width":1512,"height":1890,"filename":"1696433575844519.mp4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F70baa989-ae1f-445f-8617-8811cc70833f.mp4?alt=media&token=47c50713-aea7-4df7-a8d8-3fe22da58140","id":"70baa989-ae1f-445f-8617-8811cc70833f","width":1512,"height":1890,"filename":"1696346861012683.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2Fb51e3e74-2c3e-4fbd-bc90-ddfd5d239035.mp4?alt=media&token=f755c02f-1237-41d3-b8e6-88cf49e0734e","id":"b51e3e74-2c3e-4fbd-bc90-ddfd5d239035","width":1512,"height":1890,"filename":"1696346240021363.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2Fdb8bc437-5ccc-4638-b89f-ed6abfbdeb76.mp4?alt=media&token=fcb0f296-4bfc-460a-aae0-3c47e042216b","id":"db8bc437-5ccc-4638-b89f-ed6abfbdeb76","width":1512,"height":1890,"filename":"1696347090290536.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F0c67d695-32b6-4b2e-a267-ef2ea06fef18.mp4?alt=media&token=c695e7bc-eb4b-4a31-8908-ee6e73825515","id":"0c67d695-32b6-4b2e-a267-ef2ea06fef18","width":1512,"height":1512,"filename":"1696346581505478.MP4","type":"video/mp4","caption":"","border":false}]}
```

Nature, with its boundless beauty and ever-changing moods, serves as my endless wellspring of inspiration during outdoor shooting projects. Each venture into the great outdoors is a journey of discovery, where the play of light, the texture of landscapes, and the subtleties of the elements conspire to ignite my creativity.

The rustling leaves in a secluded forest, the dance of sunlight on a tranquil lake, or the grandeur of a rugged mountain range – all these elements come together to breathe life into my work. Nature's canvas is vast and ever-changing, providing a rich tapestry of visuals and emotions that I weave into my projects.

With each outdoor shoot, I find a deeper connection to the natural world and a renewed sense of wonder. It is in these moments, surrounded by the beauty of nature, that my artistic vision truly comes alive, resulting in visuals that resonate with depth and authenticity.

---

## **About Storytelling**

Softlove, the brand itself, placed a significant emphasis on highlighting the product's core feature:

***Comfort.***

I meticulously planned each shot to capture moments that showcased the comfort and ease that the products offer. I incorporated elements of daily life, to underscore how Softlove seamlessly integrates into one's routines, providing comfort without compromise. With the natural acting of the model, it was just the icing on the cake.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F0bf9db90-5eb4-4563-9ca2-570c6740da8a.mp4?alt=media&token=808e50f5-9bd2-40c2-92ff-37028429ad2e","id":"0bf9db90-5eb4-4563-9ca2-570c6740da8a","width":1512,"height":1890,"filename":"1696348830260479.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F77f5f8d0-3204-4e02-817d-38d111f751f5.mp4?alt=media&token=45a80883-8b3f-42fc-9de5-9df0b3ce2d8c","id":"77f5f8d0-3204-4e02-817d-38d111f751f5","width":1512,"height":1890,"filename":"1696348963601008.MP4","type":"video/mp4","caption":"","border":false}]}
```

Throughout the shoot, I adhered to a clean and visually appealing aesthetic that aligned with Softlove's brand identity. The result not only met but exceeded my expectations, effectively conveying Softlove's commitment to enhancing everyday comfort.

---

## **The final**

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsoftlove%2F7f66844d-97a5-478a-9ffc-da85d1857cf3.mp4?alt=media&token=412a01bf-8359-4df6-80a6-9b6d206132c2","id":"7f66844d-97a5-478a-9ffc-da85d1857cf3","width":1512,"height":1890,"filename":"1696397873508400.MP4","type":"video/mp4","caption":"All shots were taken using iPhone 14 Pro Max","border":true}]}
```

---

## **Credits:**

Videographer: Khor Siang Hooi\
Model: Shereen Wee\
Video Edits: Khor Siang Hooi\
\
Device: iPhone 14 Pro Max\
Applications: VSCO, VN, Unfold

Location: Perdana Botanical Garden, Malaysia

Embracing the Nature with Softlove

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F8f2bf685-970c-4e1e-803b-b2b4a4667723.mov?alt=media&token=b3e1c12d-8c98-4a4a-8f5f-93bf8e798e04","id":"8f2bf685-970c-4e1e-803b-b2b4a4667723","width":1512,"height":1890,"filename":"23722358-4F8B-4EC4-A0DF-54102721E08B.MOV","type":"video/quicktime","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F55e888a2-7142-4e42-a667-a8ba97fd7017.mov?alt=media&token=13b76213-c851-4dff-972b-de7a44c4728b","id":"55e888a2-7142-4e42-a667-a8ba97fd7017","width":1510,"height":2116,"filename":"678028DE-8797-42C0-BFCD-3640E8639065.mov","type":"video/quicktime","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fl40I5E59OowPuiusPCWt%2F65746724-fdee-4cac-bcc3-f87ad984c0f5.jpg?alt=media&token=3444ec66-96bf-4d3f-889f-1c68193296bd","id":"65746724-fdee-4cac-bcc3-f87ad984c0f5","width":2835,"height":3780,"filename":"F63EDF9D-23BD-434F-B452-1A38A1D0A280.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2Fdd049bcc-8102-47cd-88f9-09c57d89c807.mp4?alt=media&token=23a2bde7-36ce-4cfe-b199-f7bc5cce6578","id":"dd049bcc-8102-47cd-88f9-09c57d89c807","width":1512,"height":1890,"filename":"1696585437495610.MP4","type":"video/mp4","caption":"","border":false}]}
```

### **A Humble Beginning**

The world of cosmetics had always seemed like an intricate tapestry of colours, textures, and emotions to me. As a photographer and videographer, I was accustomed to capturing moments, but venturing into the realm of cosmetics photography and videography was uncharted territory. My first collaboration with Sugarbelle Cosmetics marked a pivotal moment in my career, laden with both excitement and apprehension.

It was my first time officially taking on a photography job and charging a client. The weight of the responsibility was not lost on me, and I embarked on this journey with both eagerness and nervousness. The world of cosmetics, with its lipsticks, lip creams, foundations, and eye shadows, was unfamiliar territory.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2Fabee7611-bca6-4a6f-b12a-055cfae636f3.mp4?alt=media&token=e52ec652-d43d-430a-8061-f8e1a7dd19d0","id":"abee7611-bca6-4a6f-b12a-055cfae636f3","width":1512,"height":1890,"filename":"1696622267343624.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F85723857-988f-4b4a-b2e7-966d73a86805.jpg?alt=media&token=19e65311-ccea-4d54-b6d4-95ab393fc2e3","id":"85723857-988f-4b4a-b2e7-966d73a86805","width":2844,"height":3792,"filename":"F2FED0A5-4F24-4513-9AC3-28D6EDC3A894.JPG","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2Fc2115109-fa12-4f19-a17b-239f099efb95.mp4?alt=media&token=69db8b1d-688e-4b95-9580-a5f318993ffe","id":"c2115109-fa12-4f19-a17b-239f099efb95","width":1512,"height":2688,"filename":"1696585302922845.MP4","type":"video/mp4","caption":"","border":false}]}
```

What truly astonished me was Sugarbelle's unwavering trust in my abilities. Despite this being my very first paid photography project, they believed in me and tasked me with creating 15 videos to showcase their brand. It was a daunting challenge, but their faith in my vision fueled my determination.

---

### **From Studio to Nature: Seeking Inspiration**

The quest for inspiration was a fascinating journey in itself during my collaboration with Sugarbelle Cosmetics. It was an exploration of both controlled indoor environments and the vast outdoor world, each offering its unique charm and creative challenges.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F1b01dd3a-734c-4fe7-8e30-39c950cb3f47.jpg?alt=media&token=4c9d997c-3d8d-4e0c-8201-4638e0ce3d2f","id":"1b01dd3a-734c-4fe7-8e30-39c950cb3f47","width":3024,"height":4032,"filename":"IMG_8972.jpg","type":"image/jpeg","caption":"Indoor Shots by Siang Hooi","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F4b757937-ae33-4881-8ff0-afd6a41641d2.jpg?alt=media&token=66a0662e-6791-472f-91a9-b471ed9f02d7","id":"4b757937-ae33-4881-8ff0-afd6a41641d2","width":3024,"height":4032,"filename":"tempImagezyQjtW.jpg","type":"image/jpeg","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F707b6a27-c49c-4c67-8308-1c4015b8abec.mp4?alt=media&token=954f3c64-33bc-40bf-af3f-7790e60b18f5","id":"707b6a27-c49c-4c67-8308-1c4015b8abec","width":1512,"height":2016,"filename":"1696648651117506.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F7a34c3ec-3f32-49e4-8e9e-342a10a1384e.jpg?alt=media&token=fef714a4-9d86-42a7-bb3e-8f9eddd8af5d","id":"7a34c3ec-3f32-49e4-8e9e-342a10a1384e","width":3024,"height":4032,"filename":"62464CB3-4E3E-4627-BF1D-8E44E42687E3.JPG","type":"image/jpeg","caption":"","border":false}]}
```

### **A canvas of control**

Initially, I sought creative sanctuary indoors, within the meticulously controlled confines of a studio. Here, I could orchestrate each shot with surgical precision, experimenting with lighting, angles, and compositions. The studio transformed into my canvas, where I carefully curated every detail. I was enthralled by how subtle shifts in lighting and backdrop could metamorphose a lipstick or unveil the intricate facets of an eyeshadow palette. In this realm of calculated artistry, the soft click of the camera and the gentle hum of studio lights created a symphony, as I endeavoured to capture the very essence of Sugarbelle's cosmetic marvels.

### **The Nature's Palette**

As the project unfolded, the initial wave of inspiration began to ebb, leaving me yearning for a fresh perspective. I instinctively turned to nature's grandeur, a realm where the unexpected blossomed at every turn. The natural world, with its ever-changing palette of hues and moods, beckoned me. It was time to venture beyond the studio's structured embrace.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2Fd6ad17b3-cceb-44e8-94ff-d3324521d7f7.mp4?alt=media&token=c2de26ab-57c9-49a4-b57e-ea12e0049da3","id":"d6ad17b3-cceb-44e8-94ff-d3324521d7f7","width":1512,"height":1890,"filename":"1696585848172960.MP4","type":"video/mp4","caption":"Outdoor Shots by Siang Hooi","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F23d07bed-7003-43b1-8aa3-b82dffed613a.mp4?alt=media&token=19e184bd-13fb-4e33-8c4a-a250c4b05798","id":"23d07bed-7003-43b1-8aa3-b82dffed613a","width":1512,"height":1890,"filename":"1696586808604525.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F3dee6418-3688-46d6-a690-becda728a55c.mp4?alt=media&token=dbf8a396-952d-4566-b505-2c4915fc7ade","id":"3dee6418-3688-46d6-a690-becda728a55c","width":1512,"height":1890,"filename":"1696586289318735.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2Ff248cb0e-1f07-4013-b711-82d765f5e418.mp4?alt=media&token=523e3c92-e439-4298-bf6c-fc2c7d56aa1e","id":"f248cb0e-1f07-4013-b711-82d765f5e418","width":1512,"height":2268,"filename":"1696647941454419.MP4","type":"video/mp4","caption":"","border":false}]}
```

In the embrace of nature, I found myself a humble collaborator, allowing its textures, colours, and moods to dictate the visual narrative of Sugarbelle Cosmetics. Each outdoor location gifted me a new facet of beauty, akin to an artist discovering new shades on a palette.

---

### **The Sweet Taste of Success**

As the project neared its completion, the nerves that had accompanied me at the start gave way to a sense of accomplishment. Sugarbelle was delighted with the results, and seeing my work adorn their brand was a moment of immense satisfaction.

```cv
gallery
{"layout":"hscroll","images":[{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F32013db4-60db-44f5-bf85-c00cdf58682e.mp4?alt=media&token=4120ebfa-09f7-4737-9561-c789453b82a7","id":"32013db4-60db-44f5-bf85-c00cdf58682e","width":1512,"height":2070,"filename":"1696648194301745.MP4","type":"video/mp4","caption":"","border":false},{"src":"https://firebasestorage.googleapis.com/v0/b/maitake-project.appspot.com/o/pages%2Fj5dh8CJjhNOe9Z4p7gaoRvaSgZn2%2Fsugarbelle%2F2bb6a7be-ab6c-4b87-aad1-ed8619d502ed.mp4?alt=media&token=da2f9abb-112e-49a7-9e7c-49cca0f40973","id":"2bb6a7be-ab6c-4b87-aad1-ed8619d502ed","width":1512,"height":2268,"filename":"1696650326557922.MP4","type":"video/mp4","caption":"","border":false}]}
```

This journey has taught me that venturing beyond one's comfort zone can lead to extraordinary discoveries. While I began with trepidation, I ended with a deep appreciation for the world of cosmetics photography and videography. It was not just a professional milestone but a testament to the power of inspiration, dedication, and the trust that a client can place in the hands of a passionate artist.

---

### **Credits**

Photographer/Videographer: Siang Hooi\
Model: Shereen Wee\
Video Edits: Siang Hooi

\
Device: iPhone 14 Pro Max\
Applications: VSCO, VN, Adobe Lightroom

Bridging Creativity and Cosmetics: My Sugarbelle Adventure

National Gold Award

Chinese Orchestra: 
'Chun Lei' Orchestra Competition

International Honorary Gold Award

Chinese Orchestra: 
21st Century International Arts Festival 
1 Honourary Gold Award, 3 Gold Awards, 1 Silver Award

Learn SQL Course

Artificial intelligence (AI), Generative AI

Intro to Generative AI Course

Deep Learning, Artificial Intelligence (AI), TensorFlow

Build Deep Learning Models with TensorFlow Skill Path

Learn R Course

Learn Python 3 Course

Analyse Data with SQL Skill Path

I’ve made an informed decision to pivot my academic journey. After careful consideration, I’ve transitioned to a Bachelor of Biomedical Science, aligning better with my career aspirations while staying true to my passion for medicine. Now, graduating with a Master of Bioinformatics, centered in healthcare data science, I further deepen my expertise at the intersection of medicine and AI.

Graduated with Distinction (CGPA: 4.0/4.0)

Information System, Waterfall SDLC methodology, LucidChart, Figma, UI UX

* Designed a precision agriculture system for weed management in Malaysian paddy fields, targeting users like farmers or agriculture companies.

* Applied the Waterfall SDLC methodology, covering requirement gathering, system design, and interface prototyping.

* Developed Figma mockups and system diagrams (context diagram, level diagrams, DFD, use case, etc.) in Lucidchart to visualize workflows and usability.

* Emphasized sustainable agricultural practices through efficient system design and documentation.

[View more](https://sianghooi.framer.website/work/agroplus-weed)

Concept Design: AgroPlus Weed Detection System

AI, ML/DL, Predictive Modeling, Computer Vision, PyTorch, OpenCV, Scikit-learn, Pandas, Numpy

* Implemented a deep learning framework combining U-Net for ventricular segmentation and LSTM for myocardial infarction classification.

* Processed AP4-echocardiogram videos with frame-by-frame analysis for precise feature extraction.

* Achieved 71.5% accuracy and AUC of 0.715, validated through 5-fold cross-validation.

* Optimized model performance by fine-tuning hyperparameters and leveraging advanced deep learning techniques.

* Applied AI-driven medical image analysis to enhance diagnostic accuracy in cardiac assessments.

* Established a scalable approach for future research in AI-based echocardiogram analysis by introducing this novel approach.

[View more](https://drive.google.com/file/d/1TquIC6Jtx9aETcP7ERWq4oTqyiKjg380/view?usp=drivesdk)

Deep Learning in Myocardial Infarction Detection using AP4-chamber Echocardiograms

PCR, DNA Sequencing, Cell Culture, Erythrocyte Binding Assay (EBA), Phylogenetics

* Conducted genetic analysis of Plasmodium knowlesi isolates from 2019–2021 to study polymorphisms in PkDBPαII.

* Examined samples from Peninsular Malaysia and Malaysia Borneo, with varying parasitemia levels to assess genetic diversity.

* Conducted PCR, gene sequencing, COS-7 cell line maintenance, and erythrocyte binding assays (EBA) for molecular and cellular analysis.

* Performed phylogenetic analysis, revealing no novel clustering, indicating genetic stability for recent years.

* Contributed to understanding the evolutionary dynamics of P. knowlesi in Malaysia.

[View more](https://sianghooi.framer.website/work/malaria-pkdbpaii)

Genetic Polymorphism of PkDBPaII in Malaysia

HTML, CSS, JavaScript, UI/UX, Web Development, Front-end Development

* Designed and implemented the website interface for “Sequence Space”, an unofficial gene analysis platform.

* Wrote codes fully using HTML, CSS and Javascript while deploying them at local server.

* Enhanced user experience by enabling easy analysis of palindromic sequences and DNA-to-RNA conversion.

* Prioritized intuitive design and functionality to streamline genetic data analysis.

[View more](https://sianghooi.framer.website/work/seq-space)

Creating Website Interface for Sequence Space

It's a privilege to join hands with them, a collaboration that blossomed after they recognized the quality of my previous photography endeavours.

[View more](https://forksh.framer.website/projects/wake-white)

Product Photography

I undertook the task of producing two 30-second lifestyle videos that would effectively convey the brand's commitment to enhancing everyday comfort.

[View more](https://forksh.framer.website/projects/softlove)

Short Film Creation

My work spanned indoor and outdoor settings. My role blended artistry and commerce, employing photography and videography to convey Sugarbelle's cosmetics essence and versatility.

[View more](https://forksh.framer.website/projects/sugarbelle)

Product Photography & Videography

As a concertmaster, my responsibilities extended far beyond personal musical prowess; they include the vital tasks of guiding beginners in acquiring foundational instrumental skills, fostering harmonious cooperation among all members and other school orchestras.

Concertmaster

Handling speaker logistics: Coordinating keynote presentations, assisting international speakers, and ensuring a smooth event.

Volunteer

* Gained proficiency in specimen processing, culture, microscopic examination, molecular testing, and data documentation.

* Rotated through five laboratory disciplines: Virology, Clinical Bacteriology, Mycology, Tuberculosis, and Molecular Imaging.

Laboratory Assistant

* Conducted research on the genetic polymorphism of PkDBPαII in Malaysian Plasmodium knowlesi isolates.

* Analyzed genetic variations to assess strain diversity and stability.

* Developed expertise in molecular techniques, including PCR, DNA sequencing, and phylogenetic analysis.

Undergraduate Researcher

* Specialized in product photography and videography, creating visually compelling content.

* Developed strong client relationships to enhance brand storytelling.

* Collaborated with local brands, including Sugarbelle and Softlove.

Freelance Photographer & Videographer

* Developed an AI-driven healthcare model for myocardial infarction detection using AP4-echocardiogram videos.

* Implemented U-Net semantic segmentation and LSTM architectures for accurate feature extraction and classification.

* Explored and utilized deep learning libraries, including PyTorch, OpenCV, TensorFlow, and Scikit-learn, for model development and optimization.

Khor Siang Hooi

2024

2023