## **Contact**

* **E-mail: tadej.satler@gmail.com**

* **Phone: +386 31 514 849**

* **[Website (bento)](https://bento.me/tadej)**

* **[LinkedIn](https://www.linkedin.com/in/tadejsatler/)**

* **[Twitter](https://twitter.com/TadejSatler)**

I'm a Biochemistry graduate and currently a PhD student of Biomedicine. I am passionate about Protein Design, Structural Biology, Computational Biology and Machine Learning.

## **Research work/Experience**

* Young researcher at the National Institute of Chemistry (Avg 2019 - present) ***de novo*** **Protein design • Pipelining • Computational Biology • High Performance Computing (HPC) • Protein Engineering • Artificial Intelligence (AI) • Protein purification • Protein characterization • X-ray crystallography • CryoEM**

* Internship at the University of California, San Francisco (Oct 2021 – Dec 2021) – **Integrative biology • CryoEM • Machine learning**

* Student Researcher at the University of Bristol (Erasmus+ internship) (Jan 2020 – Avg 2020) – ***de novo*** **Peptide design • Peptide purification • Peptide characterization • X-ray crystallography • Structure determination**

* Master Thesis Project at the National Institute of Chemistry (Nov 2017 – Dec 2019) – ***de novo*** **Protein design • Protein purification • Protein characterization • X-ray crystallography**

* Student Researcher at the University of Stockholm (Erasmus exchange) (Mar 2019 – Jun 2019) – **Protein purification • Protein interactions**

* Bachelor Thesis Project at the Department of Biochemistry at the Faculty of Chemistry and Chemical Technology, University of Ljubljana

## **Education**

* **University Study Programme Biochemistry**, Faculty of Chemistry and Chemical Technology, University of Ljubljana (2014-2017) o **Bachelor’s thesis** - *The effect of mutation of conserved glycine residues on the activity of cathepsin K* (Defence: 13.09.2017)

* **University Study Programme Biochemistry (Master)**, Faculty of Chemistry and Chemical Technology, University of Ljubljana (2017-2019) o **Master’s thesis** - *Preparation and characterization of bipyramidal protein cage from coiled-coils for binding of nanobodies* (Defence: 11.12.2019)

* **Interdisciplinary Doctoral Programme in Biomedicine**, Faculty of Medicine, University of Ljubljana (2020-present)

## **Awards**

* Prešern award for master thesis from Faculty of Chemistry and Chemical Technology, University of Ljubljana

CV – Tadej Satler

I am actively seeking opportunities where I can apply my expertise in Protein Design, Structural Biology, and Computational Biology, to contribute to innovative research and development projects.

Computational Biology 🧬

Grade: Average of 8.88/10
Bachelor’s thesis: The effect of mutation of conserved glycine residues on the activity of cathepsin K

Protein design, Protein Engineering, Computational Biology, Machine Learning, Protein Structure, X-ray Crystallography, CryoEM

Univeristy of Ljubljana

Grade: Average of 9.33/10
Master’s thesis: Preparation and characterization of bipyramidal protein cage from coiled-coils for binding of nanobodies

* De Novo Protein Design - Rosetta, RFdiffusion, AlphaFold2, ProteinMPNN, Modeller, MD simulations

* Structural Biology - [Cryo-electron Microscopy](https://www.rcsb.org/structure/8RVJ) , [X-ray Crystallography](https://www.rcsb.org/structure/8P4Y)

* Computational Biology

Protein Design • Pipelining • Computational Biology • High Performance Computing (HPC) • Protein Engineering • Artificial Intelligence (AI) • Protein Purification (FPLC) • Protein Characterization (CD, DLS, SEC-MALS) • PPI (ITC) • X-ray Crystallography • CryoEM

Young researcher | PhD student

Design and structure determination of novel peptide helical barrels

De Novo Peptide Design • Peptide Purification • Peptide Characterization • X-ray Crystallography • Structure Determination

Student Researcher | Intern

Helped developing deep neural networks to recognize protein residues in cryoEM densities.

Integrative Biology • CryoEM • Machine Learning • HPC • Pipelining

Developing and integrating software solutions for cryoEM data analysis. Encompassing research, testing, documentation, scripting, and web development for CryoCloud's platform.

Python • Relion • API • Docker • AWS • EC2

Tadej Satler

2023